客戶介紹:高杉,博士,教授,博士生導師,藥理學教研室主任,安徽醫科大學中青年學術技術帶頭人,美國University of Iowa訪問學者,新西蘭University of Otago短期訪問學者。研究方向:心血管藥理學。迄今指導畢業和在讀博士研究生4人,碩士研究生15人,其中6人榮獲國家研究生獎學金,2人榮獲優秀碩士論文。
科研項目:
1現代中藥新藥發現模式及關鍵技術。科技部重大新藥創制專項子課題(No 2017ZX09301-012)201701-202012,主持
2心肌爾康通過調控calpain/JP2改善橫管重構和心力衰竭,國家自然科學基金資助項目(No:81873126)201901-202212,主持
3 2017年度安徽省轉化醫學研究院科研基金項目安徽省轉化醫學研究院(No 2017zhyx40)201712-202012,主持
實驗目的:提取肝臟組織DNARNA前期實驗室處理此類組織,主要是通過手持式研磨儀處理,單次處理量只有一個,耗時,耗力。又有處理樣本的批量比較大,處理的時間較長,樣品損失比較多,難以收集等缺點。現在實驗室這邊購買使用凈信研磨儀批量處理,速度快,重復性好,解決了實驗室目前樣品難處理的情況。
設備現場照片
實驗地點、實驗材料:安徽醫科大學、肝臟組織若干,提取液,上海凈信冷凍研磨儀。
實驗步驟:
1.準備適配器,以及珠子分別放入離心管中。
2.加入珠子以及肝臟組織至研磨管。
3.將適配器裝入凈信JXSFTPRP-CL-24研磨儀中。
4.設定時間及參數,啟動儀器至研磨程序結束。
5、取出離心管,加入提取液,離心后取出研磨珠,回收后可反復利用。
實驗結果,如下圖:(圖一:研磨前樣本情況,圖二:研磨后樣本)
得到如圖實驗樣本,經跑膠對比,實驗結果較實驗室手動處理,樣本較好!
注意事項:實驗準備階段提前五分鐘開啟冷凍研磨儀,腔體降溫,防止核酸降解。
實驗所用到的儀器簡單介紹以及后續所用設備:
研磨儀:批量處理肝臟組織樣本,研磨提取核酸。
離心機:離心提取上清液。
電泳槽:跑膠分離核酸,獲取目的條帶。
凝膠成像:拍照,用來得到實驗結果
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Orbital shaker
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Standard vacuum freeze dryer
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Vertical mixer
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Non-contact ultrasonic DNA interrupter
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Rotary mixer HS-5
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Non-contact shaped DNA interrupter
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Orbital shaker
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Roller mixer (blood mixer)
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Dual-channel non-contact energy-concentrated DNA interrupter
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Rotational viscometer
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Cup type ultrasonic cell crusher
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Inching vortex oscillator
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Handheld ultrasonic cell pulverizer
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Handheld ultrasonic cell pulverizer
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Ultra-micro ultrasonic cell crusher
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Multifunctional vortex oscillator
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Rotational viscometer
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Handheld ultrasonic cell crusher
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Micro centrifuge
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Integrated ultrasonic cell crusher
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Handheld ultrasonic cell crusher
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Multi-tube vortex oscillator
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Rotational viscometer
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Micro centrifuge
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Ultrasonic crusher
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Integrated ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Ultrasonic cell crusher integrated
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Ultrasonic crusher
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Nitrogen Blowing Apparatus
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Microplate centrifuge
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Snowflake ice maker
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Integrated ultrasonic cell crusher
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Ultrasonic crusher
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Nitrogen Blowing Apparatus
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Ultrasonic crusher
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Split ultrasonic cell disruptor
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Microplate horizontal centrifuge
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Snowflake ice maker
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Ultrasonic cell crusher
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Integrated ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Round water bath nitrogen blowing instrument
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Ultrasonic cell crusher
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Dual-channel non-contact energy-concentrated DNA interrupter
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Nitrogen Blowing Apparatus
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Ultrasonic cell crusher
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Visual nitrogen blowing instrument
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Intelligent ultrasonic crusher
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Ultrasonic cell crusher
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Ultrasonic crusher
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Constant temperature airtight ultrasonic reactor
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Non-contact ultrasonic pulverizer
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Automatic sample fast grinder-24L
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Soil Ball Mill
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Microwave ultrasonic extractor
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Soil Ball Mill
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Automatic sample fast grinder-32L
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Planetary Ball Mill
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Planetary Ball Mill
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Planetary Ball Mill
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Automatic sample fast grinder-48L
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High energy grinder
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High-throughput soil grinder
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Glass bead sterilizer
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Automatic sample fast grinder-64L
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Automatic sample fast grinder-96L
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Infrared inoculation loop sterilizer
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Hair drug detector
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Infrared inoculation loop sterilizer
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Hair screening drug detector
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Hair treatment instrument
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Portable hair grinder
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Biological indicator incubator
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Hair Grinding and Shredder
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Multi-sample tissue grinding machine-FEIII
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Multi-sample tissue grinder-FEII
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Tissue grinder
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Tissue grinding machine
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Plant tissue processing system
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Multi-sample tissue grinding machine-192
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Multi-sample tissue grinder-96
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Multi-sample tissue grinder-64
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Multi-sample tissue grinder -48
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Multi-sample tissue grinding machine-32
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Multi-sample tissue grinder-24
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QuEChERS pre-processing all-in-one machine
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QuEChERS sample preparation system
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流體實驗魯爾轉接頭
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細胞定位格子培養皿
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Thermostatic metal bath
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μ-Dish 35 mm, high Grid-50 Glass Bottom
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Thermostatic metal bath
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Thermostatic metal bath
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Mini refrigerated metal bath
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Mini thermostat metal bath
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六通道載玻片
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Thermostatic metal bath refrigeration type
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μ-Dish 35 mm, high tissue culture dish
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In situ hybridization
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8孔腔室載玻片
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Multifunctional cell electric fusion instrument
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UV crosslinker
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細胞定位格子培養皿
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12孔可移除腔室載玻片
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六通道載玻片0.1
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魯爾固定連接器-F
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魯爾固定接頭-M
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魯爾公接頭
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魯爾公插頭
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Molecular hybridization furnace
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CultureWell?可重復使用硅膠墊片
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內毒素檢測
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TH-5熱敏性溫度監測儀
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BAT-12微探針溫度計
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BAT-10 多功能溫度計
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灌注管道裝置
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灌注槽
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灌注設備
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2孔腔室載玻片-無凹液面
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18孔培養載玻片-扁平版
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API Coryne
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常規培養基
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CoverWell?成像小室
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成像墊片(imaging spacer)
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一體化成像系統 (具備AI人工智能技術)
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High temperature four-module thermostatic metal bath
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熒光蛋白激發觀察系統
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熒光蛋白激發觀察系統
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便攜式顯微鏡-帶XY工作臺
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PM-1 便攜式顯微鏡
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細胞拉伸系統
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Culture Well?可移除腔室蓋玻片
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其他設備(電生理/IVF)
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SecureSlip?帶硅膠層蓋玻片
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活細胞培養系統
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Three-dimensional mixer
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磁石培養皿
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CoverWell?灌流小室
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CultureWell?多孔腔室蓋玻片
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共培養載玻片
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磁座組合式活細胞培養槽
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3D Centrifugal Cryogenic Grinder
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Small bead mill/cell bead mill
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Automatic sample rapid grinder-192
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Cryogenic Grinder JXFSTPRP-CLN
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Cryogenic tissue grinder
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Cryogenic tissue grinder
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High-speed cryogenic grinding homogenizer
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Multi-sample tissue grinder-24L
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High-speed tissue grinding machine KZ-II
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Multi-channel microbial magnetic bead homogenizer
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Automatic sample rapid grinding machine
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Automatic sample rapid grinder-24
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Immersion liquid nitrogen Cryogenic Grinder
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Automatic liquid nitrogen freezing grinding machine
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Automatic sample rapid grinding machine-64
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Automatic sample rapid grinding machine-48
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Constant temperature and humidity weighing system
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Vertical oscillator
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Multi-sample tissue grinder-192L
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Multi-sample tissue grinder-96L
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Multi-sample tissue grinder-64L
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Cryogenic Grinder (Semi-automatic Liquid Nitrogen Cryogenic Grinder)
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Multi-sample tissue grinder-32L
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3D sample tissue grinder
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Automatic sample rapid grinder-32
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Automatic sample rapid grinding machine
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Fully automatic sample rapid cryogenic grinder
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Fast tissue low-temperature crushing homogenizer
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Cryogenic Grinding Machine
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QuEChERS pre-processing all-in-one machine
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Multi-sample tissue grinder-48L
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96孔板
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High energy ball mill
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3D Cryogenic Grinder
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3D Centrifugal Cryogenic Grinder
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Cryogenic tissue grinder
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Frozen grinder
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Cryogenic grinder
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Basic cryogenic grinder
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Frozen Bone Teeth Crusher
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Mini grinder
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Mini Diamond Grinding Machine
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MINI rapid detection grinder
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Portable sample grinder
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Portable sample grinder
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Automatic sample rapid grinder-96
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Chlorophyll A grinder
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Semi-automatic hammer cyclone mill
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Automatic hammer cyclone mill
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Low temperature hammer type cyclone mill
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Multifunctional homogenizer
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Three-head shock type high-energy ball mill
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瓊脂糖
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Water quality chlorophyll grinder
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Three-dimensional mixer
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Mortar grinder
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High-efficiency vibrating disc grinder
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Fully automatic cryogenic sample processing system
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Automatic sample rapid grinder
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Three-dimensional mixer
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Hot-lid metal bath
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Three-dimensional high-speed vibration ball mill
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NTE-2A溫度探針&控制器
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Automatic homogenizer
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Beating sample homogenizer
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Solid phase extraction device
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Solid Phase Extraction Apparatus
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組織切片全系統
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High temperature type CNC super constant temperature bath
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Vibratron II振動型整流器
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Automatic rotating sampler
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Aquatic product animal husbandry solid phase extraction device
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Knife grinder
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培養皿載玻片保濕盒
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玻璃底培養皿
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串聯連接管
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Biological indicator incubator
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彈性底部培養皿
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Beating aseptic homogenizer
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4孔腔室載玻片-無凹液面
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Oil-free vacuum pump
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WF-1內置沉淀過濾器
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細胞分離機附屬冷凍系統
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TCAT-2 動物溫度控制器
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MTC-1微型溫度控制器
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定制熱電偶
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加熱毯WB-1, WB-2
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TCAT-2配件: 加熱燈HL-1
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加熱板HP-1M & HP-4M
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Cross-beating grinder
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交換器SWT-2
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Single cell suspension preparation instrument
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水泵和水箱裝置
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TS-4MP恒溫顯微鏡載物臺
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Sample pre-processing machine
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Fully automatic homogenizer
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Beating aseptic homogenizer
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Grinding bead homogenizer
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Parallel grinder
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交換器SBT-5
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細胞趨化載玻片3D
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Jaw Crusher
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ibidi Pump System
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In-line Luer Injection Port
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μ-Plate Angiogenesis 96 Well
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μ-Slide y-shaped
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Constant temperature mixer (refrigeration)
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Constant temperature mixer
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μ-Slide I Luer Glass Bottom
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Culture-Insert 2 Well in μ-Dish 35 mm
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μ-Slide 8 Well high
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2D細胞趨化載玻片
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μ-Slide 15 Well 3D (formerly μ-Slide Angiogenesis)
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μ-Slide I
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μ-Dish Microscopy Rack
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sticky-Slide Chemotaxis
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Hose Clip
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Custom-Specific Flow Slides and Channels
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Dry thermostat
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減管徑配件
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微4孔插件
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Single cell preparation instrument (temperature control)
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Microplate Shaker
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Microplate incubator
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Microplate incubator
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Oscillator special for microplate
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硅膠管
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μ-Slide 18 Well
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Microplate Heat Sealer
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器官運輸器
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單通道載玻片
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三合一通道載玻片
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六通道平版載玻片
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單通道可粘載玻片
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六通道可粘載玻片
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Temperature-controlled single cell suspension preparation instrument
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μ-Slide I Luer 3D
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Microplate Oscillator
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梯度混勻器
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Ricordi? 注射袋
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High Power Handheld Grinder
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Bone and Teeth Grinder
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溫度顯示器
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溫度探針
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溫度探針延長線
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胰島分離機
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Handheld Grinder
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分離管道裝置
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Fully automatic low temperature flip oscillator
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API 20 E
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API 20 NE
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API 20 STAPH
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API 20 A
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API NH
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API Listeria
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API RapiD 20E
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Ricordi? 腔室系統
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Y型連接管
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無血清培養基
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細胞培養試劑
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原代細胞及推薦培養基
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支原體檢測
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轉染試劑
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8孔可粘腔室載玻片
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μ-Dish 35 mm, low
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New type soil drying box
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24孔板
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API ZYM/ API 50CHB/ API 50CHL
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2孔腔室載玻片
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4孔腔室載玻片
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Gradient amplification PCR detection analyzer THG-96G (gradient)
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Gene amplification instrument PCR instrument THT-96G (gradient type)
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High-speed grinding homogenizer-192
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Flip oscillator
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Fully Automatic Flip Oscillator
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DIC培養皿蓋/載玻片蓋
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胰島計數皿
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High-throughput sample rapid grinder
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Medium Flux Grinder
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High Throughput Grinder
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High Throughput Tissue Grinder-2018
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High Throughput Tissue Grinder - GTL64
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氮化硅彈珠
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細胞抽樣管
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菌種保存管
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胰島細胞計數儀
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T型熱電偶金屬線
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生物流體灌流系統
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灌流小室
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灌流管道裝置
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灌流噴頭
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灌流腔室過濾器
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COBE? 2991 血細胞淘洗機
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閃膠
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BNC熱電偶
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通用型探針
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胰島運輸器
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API Campy
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TH-8 熱敏性檢測溫度計
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BAT7001H溫度計
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THERMES USB 溫度數據采集
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THERMES USB WFI 溫度數據采集
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針型微探針
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Ultra High Throughput Tissue Grinder
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超細IT-系列靈活探針
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Ultra High Throughput Tissue Grinder
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臨床探針
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動物直腸探針
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RET-2-ISO
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RET-3-ISO
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RET-4直腸探針
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IT系列特氟龍探針
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MT系列針型微探針
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API 10S
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靈活可植入探針
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Grinder for scientific research
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Grinder for analysis?and?detection
Grinder for analysis?and?detection
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General?Instruments
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Xiaomei Ultrasound Series
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