客戶簡介:*實驗動物中心是一所綜合性大學重要的公共技術服務平臺,中心同時設有動物胚胎實驗區、動物細胞實驗區,并配備專業技術人員,可提供胚胎凈化、保種等多項技術服務。
課題組介紹:
劉林教授主持了多項國家級重大項目,包括國家重點研究計劃項目、國家重大科學研究計劃項目、國家重大轉基因專項項目、國家國際科技合作專項項目、國家自然科學基金委重點及重大研究計劃項目等。
研究方向:異多能性干細胞重編程和轉基因研究,哺乳動物早期發育的分子機制
實驗目的:小鼠肝臟組織提取DNA,因手工研磨費時費力,影響提取效果,因此選擇上海凈信多樣品組織研磨儀。
實驗材料和器具:
樣品:小鼠肝臟組織
工具:上海凈信多樣品組織研磨儀(Tissuelyser-24)
耗材:離心管,研磨珠、鋼珠、液氮、剪刀等
實驗步驟:
1、準備離心管,研磨珠、研磨珠;
2、首先將將適配器放入冰箱冷凍幾分鐘,以便在研磨過程中保持樣品低溫狀態,保證樣品活性;
3、取小鼠肝臟組織、剪成小塊與研磨鋼珠分別放入離心管內、加入溶液,將蓋子扣緊;
4、將適配器放入研磨儀中固定,然后在將鋼罐放置適合器內。樣品盡量對稱放置,以保證研磨時的受力平衡,旋緊垂直定位螺旋,將安全鎖扣緊。設置頻率及時間3,程序結束后即可得到破碎漿液;
5、研磨完畢后請關閉電源,將安全鎖歸位;
6、完成后續DNA提取步驟。
注意事項:
1、每支離心管樣品量一般不超過放心管體積的三分之二,樣品量過多會影響研磨效果。
2、樣品盡量對稱放置,以保證研磨時的受力平衡。
3、適配器采用半導體材質需要提前放入冰箱冷凍幾分鐘。
實驗過程、結果圖示:
實用儀器介紹:
此款儀器為上海凈信實業發展有限公司生產的全新一代Tissuelyser®組織研磨儀,采用進口電機,防腐材質,增強型運動軸等多種提升??梢栽谳^短的時間內完成對各種樣品的研磨、粉碎、混合及細胞破壁。
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Orbital shaker
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Standard vacuum freeze dryer
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Vertical mixer
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Non-contact ultrasonic DNA interrupter
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Rotary mixer HS-5
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Non-contact shaped DNA interrupter
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Orbital shaker
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Roller mixer (blood mixer)
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Dual-channel non-contact energy-concentrated DNA interrupter
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Rotational viscometer
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Cup type ultrasonic cell crusher
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Inching vortex oscillator
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Handheld ultrasonic cell pulverizer
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Handheld ultrasonic cell pulverizer
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Ultra-micro ultrasonic cell crusher
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Multifunctional vortex oscillator
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Rotational viscometer
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Handheld ultrasonic cell crusher
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Micro centrifuge
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Integrated ultrasonic cell crusher
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Handheld ultrasonic cell crusher
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Multi-tube vortex oscillator
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Rotational viscometer
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Micro centrifuge
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Ultrasonic crusher
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Integrated ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Ultrasonic cell crusher integrated
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Ultrasonic crusher
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Nitrogen Blowing Apparatus
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Microplate centrifuge
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Snowflake ice maker
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Integrated ultrasonic cell crusher
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Ultrasonic crusher
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Nitrogen Blowing Apparatus
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Ultrasonic crusher
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Split ultrasonic cell disruptor
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Microplate horizontal centrifuge
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Snowflake ice maker
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Ultrasonic cell crusher
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Integrated ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Round water bath nitrogen blowing instrument
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Ultrasonic cell crusher
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Dual-channel non-contact energy-concentrated DNA interrupter
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Nitrogen Blowing Apparatus
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Ultrasonic cell crusher
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Visual nitrogen blowing instrument
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Intelligent ultrasonic crusher
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Ultrasonic cell crusher
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Ultrasonic crusher
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Constant temperature airtight ultrasonic reactor
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Non-contact ultrasonic pulverizer
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Automatic sample fast grinder-24L
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Soil Ball Mill
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Microwave ultrasonic extractor
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Soil Ball Mill
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Automatic sample fast grinder-32L
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Planetary Ball Mill
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Planetary Ball Mill
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Planetary Ball Mill
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Automatic sample fast grinder-48L
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High energy grinder
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High-throughput soil grinder
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Glass bead sterilizer
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Automatic sample fast grinder-64L
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Automatic sample fast grinder-96L
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Infrared inoculation loop sterilizer
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Hair drug detector
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Infrared inoculation loop sterilizer
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Hair screening drug detector
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Hair treatment instrument
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Portable hair grinder
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Biological indicator incubator
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Hair Grinding and Shredder
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Multi-sample tissue grinding machine-FEIII
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Multi-sample tissue grinder-FEII
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Tissue grinder
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Tissue grinding machine
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Plant tissue processing system
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Multi-sample tissue grinding machine-192
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Multi-sample tissue grinder-96
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Multi-sample tissue grinder-64
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Multi-sample tissue grinder -48
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Multi-sample tissue grinding machine-32
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Multi-sample tissue grinder-24
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QuEChERS pre-processing all-in-one machine
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QuEChERS sample preparation system
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流體實驗魯爾轉接頭
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細胞定位格子培養皿
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Thermostatic metal bath
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μ-Dish 35 mm, high Grid-50 Glass Bottom
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Thermostatic metal bath
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Thermostatic metal bath
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Mini refrigerated metal bath
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Mini thermostat metal bath
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六通道載玻片
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Thermostatic metal bath refrigeration type
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μ-Dish 35 mm, high tissue culture dish
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In situ hybridization
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8孔腔室載玻片
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Multifunctional cell electric fusion instrument
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UV crosslinker
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細胞定位格子培養皿
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12孔可移除腔室載玻片
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六通道載玻片0.1
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魯爾固定連接器-F
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魯爾固定接頭-M
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魯爾公接頭
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魯爾公插頭
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Molecular hybridization furnace
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CultureWell?可重復使用硅膠墊片
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內毒素檢測
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TH-5熱敏性溫度監測儀
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BAT-12微探針溫度計
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BAT-10 多功能溫度計
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灌注管道裝置
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灌注槽
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灌注設備
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2孔腔室載玻片-無凹液面
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18孔培養載玻片-扁平版
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API Coryne
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常規培養基
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CoverWell?成像小室
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成像墊片(imaging spacer)
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一體化成像系統 (具備AI人工智能技術)
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High temperature four-module thermostatic metal bath
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熒光蛋白激發觀察系統
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熒光蛋白激發觀察系統
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便攜式顯微鏡-帶XY工作臺
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PM-1 便攜式顯微鏡
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細胞拉伸系統
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Culture Well?可移除腔室蓋玻片
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其他設備(電生理/IVF)
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SecureSlip?帶硅膠層蓋玻片
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活細胞培養系統
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Three-dimensional mixer
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磁石培養皿
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CoverWell?灌流小室
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CultureWell?多孔腔室蓋玻片
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共培養載玻片
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磁座組合式活細胞培養槽
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3D Centrifugal Cryogenic Grinder
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Small bead mill/cell bead mill
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Automatic sample rapid grinder-192
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Cryogenic Grinder JXFSTPRP-CLN
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Cryogenic tissue grinder
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Cryogenic tissue grinder
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High-speed cryogenic grinding homogenizer
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Multi-sample tissue grinder-24L
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High-speed tissue grinding machine KZ-II
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Multi-channel microbial magnetic bead homogenizer
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Automatic sample rapid grinding machine
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Automatic sample rapid grinder-24
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Immersion liquid nitrogen Cryogenic Grinder
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Automatic liquid nitrogen freezing grinding machine
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Automatic sample rapid grinding machine-64
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Automatic sample rapid grinding machine-48
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Constant temperature and humidity weighing system
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Vertical oscillator
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Multi-sample tissue grinder-192L
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Multi-sample tissue grinder-96L
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Multi-sample tissue grinder-64L
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Cryogenic Grinder (Semi-automatic Liquid Nitrogen Cryogenic Grinder)
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Multi-sample tissue grinder-32L
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3D sample tissue grinder
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Automatic sample rapid grinder-32
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Automatic sample rapid grinding machine
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Fully automatic sample rapid cryogenic grinder
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Fast tissue low-temperature crushing homogenizer
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Cryogenic Grinding Machine
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QuEChERS pre-processing all-in-one machine
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Multi-sample tissue grinder-48L
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96孔板
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High energy ball mill
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3D Cryogenic Grinder
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3D Centrifugal Cryogenic Grinder
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Cryogenic tissue grinder
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Frozen grinder
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Cryogenic grinder
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Basic cryogenic grinder
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Frozen Bone Teeth Crusher
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Mini grinder
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Mini Diamond Grinding Machine
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MINI rapid detection grinder
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Portable sample grinder
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Portable sample grinder
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Automatic sample rapid grinder-96
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Chlorophyll A grinder
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Semi-automatic hammer cyclone mill
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Automatic hammer cyclone mill
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Low temperature hammer type cyclone mill
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Multifunctional homogenizer
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Three-head shock type high-energy ball mill
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瓊脂糖
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Water quality chlorophyll grinder
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Three-dimensional mixer
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Mortar grinder
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High-efficiency vibrating disc grinder
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Fully automatic cryogenic sample processing system
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Automatic sample rapid grinder
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Three-dimensional mixer
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Hot-lid metal bath
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Three-dimensional high-speed vibration ball mill
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NTE-2A溫度探針&控制器
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Automatic homogenizer
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Beating sample homogenizer
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Solid phase extraction device
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Solid Phase Extraction Apparatus
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組織切片全系統
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High temperature type CNC super constant temperature bath
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Vibratron II振動型整流器
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Automatic rotating sampler
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Aquatic product animal husbandry solid phase extraction device
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Knife grinder
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培養皿載玻片保濕盒
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玻璃底培養皿
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串聯連接管
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Biological indicator incubator
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彈性底部培養皿
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Beating aseptic homogenizer
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4孔腔室載玻片-無凹液面
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Oil-free vacuum pump
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WF-1內置沉淀過濾器
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細胞分離機附屬冷凍系統
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TCAT-2 動物溫度控制器
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MTC-1微型溫度控制器
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定制熱電偶
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加熱毯WB-1, WB-2
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TCAT-2配件: 加熱燈HL-1
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加熱板HP-1M & HP-4M
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Cross-beating grinder
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交換器SWT-2
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Single cell suspension preparation instrument
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水泵和水箱裝置
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TS-4MP恒溫顯微鏡載物臺
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Sample pre-processing machine
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Fully automatic homogenizer
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Beating aseptic homogenizer
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Grinding bead homogenizer
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Parallel grinder
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交換器SBT-5
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細胞趨化載玻片3D
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Jaw Crusher
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ibidi Pump System
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In-line Luer Injection Port
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μ-Plate Angiogenesis 96 Well
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μ-Slide y-shaped
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Constant temperature mixer (refrigeration)
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Constant temperature mixer
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μ-Slide I Luer Glass Bottom
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Culture-Insert 2 Well in μ-Dish 35 mm
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μ-Slide 8 Well high
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2D細胞趨化載玻片
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μ-Slide 15 Well 3D (formerly μ-Slide Angiogenesis)
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μ-Slide I
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μ-Dish Microscopy Rack
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sticky-Slide Chemotaxis
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Hose Clip
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Custom-Specific Flow Slides and Channels
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Dry thermostat
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減管徑配件
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微4孔插件
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Single cell preparation instrument (temperature control)
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Microplate Shaker
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Microplate incubator
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Microplate incubator
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Oscillator special for microplate
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硅膠管
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μ-Slide 18 Well
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Microplate Heat Sealer
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器官運輸器
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單通道載玻片
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三合一通道載玻片
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六通道平版載玻片
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單通道可粘載玻片
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六通道可粘載玻片
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Temperature-controlled single cell suspension preparation instrument
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μ-Slide I Luer 3D
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Microplate Oscillator
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梯度混勻器
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Ricordi? 注射袋
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High Power Handheld Grinder
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Bone and Teeth Grinder
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溫度顯示器
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溫度探針
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溫度探針延長線
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胰島分離機
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Handheld Grinder
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分離管道裝置
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Fully automatic low temperature flip oscillator
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API 20 E
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API 20 NE
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API 20 STAPH
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API 20 A
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API NH
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API Listeria
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API RapiD 20E
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Ricordi? 腔室系統
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Y型連接管
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無血清培養基
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細胞培養試劑
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原代細胞及推薦培養基
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支原體檢測
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轉染試劑
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8孔可粘腔室載玻片
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μ-Dish 35 mm, low
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New type soil drying box
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24孔板
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API ZYM/ API 50CHB/ API 50CHL
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2孔腔室載玻片
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4孔腔室載玻片
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Gradient amplification PCR detection analyzer THG-96G (gradient)
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Gene amplification instrument PCR instrument THT-96G (gradient type)
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High-speed grinding homogenizer-192
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Flip oscillator
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Fully Automatic Flip Oscillator
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DIC培養皿蓋/載玻片蓋
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胰島計數皿
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High-throughput sample rapid grinder
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Medium Flux Grinder
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High Throughput Grinder
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High Throughput Tissue Grinder-2018
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High Throughput Tissue Grinder - GTL64
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氮化硅彈珠
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細胞抽樣管
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菌種保存管
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胰島細胞計數儀
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T型熱電偶金屬線
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生物流體灌流系統
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灌流小室
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灌流管道裝置
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灌流噴頭
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灌流腔室過濾器
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COBE? 2991 血細胞淘洗機
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閃膠
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BNC熱電偶
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通用型探針
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胰島運輸器
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API Campy
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TH-8 熱敏性檢測溫度計
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BAT7001H溫度計
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THERMES USB 溫度數據采集
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THERMES USB WFI 溫度數據采集
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針型微探針
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Ultra High Throughput Tissue Grinder
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超細IT-系列靈活探針
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Ultra High Throughput Tissue Grinder
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臨床探針
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動物直腸探針
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RET-2-ISO
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RET-3-ISO
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RET-4直腸探針
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IT系列特氟龍探針
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MT系列針型微探針
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API 10S
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靈活可植入探針
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Product
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Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
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Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
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General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
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Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
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Vibrating?sieve?shakers
Vibrating?sieve?shakers
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Foreign distribution products
Foreign distribution products
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Grinder for scientific research
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