客戶簡介:
中南民族大學位于湖北省武漢市,是中華人民共和國國家民族事務委員會直屬高校,為中國“少數民族骨干計劃”資格高校、全國深化創新創業教育改革示范高校、中國高校行星科學聯盟、湖北省“國內一流大學建設高校”。
實驗目的:
檢測藻細胞內總蛋白含量和抗氧化酶活性
實驗材料和器具:
樣品:微囊藻7806 (Microcystis aeruginosa PCC 7806)
工具:小美超微量超聲波細胞破碎儀(XM-650T)
耗材:10ml離心管
實驗步驟:
1.準備小美超聲波破碎儀,配加長變幅桿
2.將探頭放入準備好的樣品中處理中
3.超聲條件設置
4.具體實驗流程:取20ml集胞藻6803藻液(OD0.6-0.8),常溫離心,棄上清。沉淀加2ml的tris-HCl(40mM, PH8.0)及20ul的PMSF(100mM),超聲處理。超聲后,4度離心,取上清轉移至新的離心管,用于蛋白定量和抗氧化酶活性檢測。
注意:
1、每次破碎樣品時盡量避免探頭貼壁,也可根據客戶自己操作調節
2、由于連續超聲破碎,會使樣品溫度升高,需要客戶冰浴處理
實驗過程、結果圖示:
XM-650T
圖1破碎前,圖2破碎后
此款儀器為上海凈信實業發展有限公司旗下小美超聲生產的全新超聲波破碎儀一體機,是一種特殊的、快速的、高效率的一體機破碎系統。可以在較短的時間內完成對各種樣品的研磨、粉碎、混合及細胞破壁,以保留樣品活性。
產品簡介:
1、藥物提取,細胞,細菌,病毒組織破碎。如細胞內含物的萃取。
2、物質顆粒的分散、勻質,及乳化。如納米材料的分散。
3、加速溶解,加速化學反應。如用于化學合成,此超聲設備配隔音箱支架、專用隔音箱、低溫系統。
4、超聲破碎是聲化學設備的一種應用,可用于植物提取,水處理、固液系分散、液體中顆粒的解團聚、促進固液反應等效果,使較大粒徑的聚集體分散為較小的顆粒。穩定化指保證粉體顆粒在液體中保持長期的均勻分散等。
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