客戶介紹:浙江農林大學位于杭州市臨安區、是浙江省重點建設高校、浙江省人民政府與國家林業和草原局共建高校。學校以農林、生物、環境學科為特色,涵蓋八大學科門類的多科性大學。
實驗目的:愈傷組織(callus)是指原植物體的局部受到創傷刺激后,在傷口表面新生的組織。它由活的薄壁細胞組成,可起源于植物體任何器官內各種組織的活細胞。本次實驗對象為愈傷組織,要求磨細,而且凍液氮磨,后續用來提RNA。
實驗步驟:
1、準備JXFSTPRP-24L組織研磨儀一臺、離心管、氧化鋯研磨珠以及所需研磨愈傷樣品若干。
2、取適量愈傷組織、一顆氧化鋯研磨珠一起放入離心管中,蓋子蓋上。
3、將配備好的離心管放入液氮中預冷,凍脆樣品。金屬適配器底座也一起放入液氮預冷(預冷2-3分鐘)
4、將離心管適配器放入研磨儀中固定(適配器底部凹槽與軸的卡槽互相卡牢)放平后將離心管分別放入適配器對應孔位,(注意平面配平,對稱放樣)蓋上適配器蓋,旋緊螺帽。
5、設置參數(根據所需研磨量微調時間),開始研磨。
6、磨好后打開儀器取出離心管,即可進行后續實驗。
(愈傷組織) (裝管圖)
(研磨效果圖) (取樣圖)
上圖為1分鐘的研磨效果,已經完全研磨完成。效果非常好,老師要求愈傷研磨一定要凍液氮,否則rna可能降解,所以人工研磨特別不方便,經過多次了解和對比所以老師們選用凈信研磨儀代替人工處理。效率高,效果好。完美解決了老師的研磨問題。
注意事項:
1、液氮操作需佩戴操作液氮的厚橡膠手套,注意安全。
2、放研磨管時時注意平面配平,只有一個樣品時需用空管配平。
3、凍液氮過程中一定是裝好樣品和珠子關蓋的研磨管,不要開蓋浸入液氮,液氮發熱膨脹容易爆管。
實驗選用儀器: 全自動樣品快速研磨儀 JXFSTPRP-24L
- Home
- About us
-
Product
-
Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
-
Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
-
General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
-
Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
-
Vibrating?sieve?shakers
Vibrating?sieve?shakers
-
Foreign distribution products
Foreign distribution products
-
Grinder for scientific research
- Customers
- News
- Videos
- Case
- Cantact