樟樹是一種用途很廣的樹種,在我國資源非常豐富,藥用價值和綠化價值非常高。主要分布于我國南方各省,尤以福建臺灣為最多。具有祛風,除濕,解毒,殺蟲之功效。主治風濕痹痛,胃痛,水火燙傷,瘡瘍腫毒,慢性下肢潰瘍,疥癬,皮膚瘙癢等。
近年來,國內外學者在樟樹的化學成分和生物活性方面做了一些研究。研究發現樟樹含有化學成分主要包括揮發性油、生物堿類、黃酮類、有機酸等。并且發現樟樹的莖、葉、根樟樹籽等各部分提取物具有一定的生物活性,主要涉及抑菌、驅蟲、抗氧化、消炎等方面。
然而,這些方面的研究還不夠系統,有必要對樟樹葉的化學成分和生物活性進行更系統全面的研究。
下面內容講述廣東省林科院科研團隊研磨樟樹葉片前處理的方法步驟:
一、實驗儀器多樣品組織研磨儀Tissuelyser-48L(下圖所示)
二、上海凈信®的Tissuelyser®組織研磨儀(專利產品,仿冒必究!ZL2019301641779)是一種特殊的、快速的、高效率的、多試管的一致系統。它能將任何來源(包括土壤、植物和動物的組織/器官、細菌、酵母、真菌、孢子、古生物標本等)的原始DNA、RNA和蛋白質進行提取和純化。Tissuelyser-48L采用垂直振蕩方式,研磨更充分,1分鐘內完成2×24、2×48、4×96個樣品的研磨;同一組織樣本設定相同程序,獲得相同的研磨效果;內置程序控制器,可對研磨時間、轉子的振動頻率等參數進行設置;具備安全罩及安全鎖;無交叉污染且低噪音。
實驗步驟:
將樟樹葉片剪小段后放入若干ML研磨罐中,加入特定規格的研磨介質,然后把研磨罐放入液氮眾泡制幾分鐘,泡制至超低溫后放進上海凈信多樣品研磨儀內,設置好研磨儀工作時間和頻率,最后啟動設備。
實驗結果:
Previous:全自動組織研磨儀|研磨牙齒從牙齒中提取DNA
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