實驗背景
大、小鼠皮膚提蛋白目前是各高校動物實驗室的一項難題,目前客戶用勻漿器手動研磨方法操作麻煩,溫度控制難度大,蛋白容易在研磨過程中因瞬時溫度和研磨顆粒較大,無法做出好的實驗效果。上海凈信研磨儀根據客戶要求提供技術支持,解決華中科技大學生科院某實驗室技術難題。
某生物實驗交流網站帖子詢問WB解決方案,無準確回答解決方案。
實驗解決方案:
1.儀器配置:上海凈信多樣品組織研磨儀一臺,模塊一套。
研磨儀主機/5ml不銹鋼罐適配器
2.實驗器材需求:適量大小保溫瓶一個,眼科剪,鑷子,鑰勺,后續配套離心管、裂解液。
皮膚去脂肪層鋪展開效果/ 取材,準備放入適配器液氮冷凍
將皮膚放入5ml不銹鋼罐,剪碎至一定程度,放入鋼珠,然后一同放入保溫瓶中冷凍至不沸騰,大概3分鐘左右將適配器一同快速裝入研磨機中,調好頻率和時間研磨。研磨完成后將組織轉入EP管中用裂解液溶解。
皮膚研磨后效果圖/加入裂解液后效果圖
研磨完成后進行體蛋白提取及后續實驗,此方法最小通量為24個2mlEP管。大大提高了實驗人員的工作效率,并且研磨時間短并且均衡,能控制好全程溫度在-80℃以下。
總結:上海凈信研磨儀對皮膚及動、植物或各種材料標本有全套解決方案,高通量地解決實驗重難點問題,讓實驗更加輕松快捷,省去大量人力物力,深受各高校喜愛。
Previous:多樣品組織研磨儀研磨豬皮實驗-上海凈信
Related Products
- Home
- About us
-
Product
-
Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
-
Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
-
General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
-
Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
-
Vibrating?sieve?shakers
Vibrating?sieve?shakers
-
Foreign distribution products
Foreign distribution products
-
Grinder for scientific research
- Customers
- News
- Videos
- Case
- Cantact