近年來,隨著生命科學研究的發展,尤其是在完成擬南芥 (Arabidopsis thaliana) 和水稻 (Oryza sativa) 等植物的基因組測序后,植物生物學發生了翻天覆地的變化。人們已經把目光從基因的測序轉移到了基因的功能研究。在研究 DNA 的基因組學、mRNA 的轉錄組學及蛋白質的蛋白組學后,接踵而來的是研究代謝物的代謝組學 (Hall et al.,2002)。代謝組學的概念來源于代謝組,代謝組是指某一生物或細胞在一特定生理時期內所有的低分子量代謝產物,代謝組學則是對某一生物或細胞在一特定生理時期內所有低分子量代謝產物同時進行定性和定量分析的一門新學科 (Goodacre,2004)。它是以組群指標分析為基礎,以高通量檢測和數據處理為手段,以信息建模與系統整合為目標的系統生物學的一個分支。
實驗步驟:
步驟1:將采集的各種植物組織(根莖葉,根瘤菌等)裝入研磨管,加入研磨珠。放入液氮中進行低溫處理。
步驟2:將低溫處理過的樣品放入凈信研磨儀,設置頻率,研磨完成后可以得到粉末狀的樣品
步驟3:從凈信研磨儀中取出樣品,將樣品溶解做后續實驗
實驗結果
研磨效果
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