1)時間:2012/7/6 注:部分請與廠家工程師聯系
2)樣品:礦石
3)儀器:主機,Tissuelyser全自動組織研磨器(上海凈信實業有限公司生產)
4)配件:鋼罐和研磨珠(上海凈信實業有限公司生產)
4)步驟:
A.取一些準備好的礦石樣本,把他們分割成幾小塊,分別放入鋼罐中。
B.每個鋼罐中在放入幾顆凈信的研磨珠,分別將鋼罐旋好后,再置入研磨適配器中,將適配器固定在研磨儀中。
C.然后去設置Tissuelyser全自動組織研磨儀的頻率和時間參數,設置好之后啟動研磨儀。
D.研磨完全停止后,拿出適配器上的鋼罐,旋下鋼罐蓋子,取出研磨好的礦石樣品。
E.然后取出研磨成細粉的礦石樣本將其放入凈信的離心管中,在離心管中加入幾微升的裂解液,用于下一步的實驗。
6)結果:
由圖可以清晰的看出,研磨后的土壤很均勻的細粉狀,說明用Tissuelyser全自動組織研磨儀研磨土壤效果非常良好,可以用于后續的礦石成分分析。
上海凈信實業發展有限公司,是目前國內一家生產水平式組織研磨機,垂直式組織研磨機,三維一體式組織研磨機具有全系列生產線的生產廠家。同時還給多家國外品牌及國內企業提供OEM,貼牌代加工服務。
上海凈信的全自動樣品快速研磨儀 處理與手工液氮研磨處理后提取 FT 的 DNA、RNA 在濃度、純度上均無顯著性差異 。但手工研磨有較大的樣本損失率,對于DNA提取濃度較低的樣品來說,自動研磨儀可以做到樣品零損失,在DNA提取上有著明顯的優勢,且手工處理的過程復雜、易交叉污染,操作所用時間等方面均不及上海凈信的全自動樣品快速研磨儀,因此自動研磨儀對于DNA提取工作相對于手工研磨處理有很大優勢。
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